Your headcanons

Hard to be trans when your species doesn't seem to have separate sexes anyways. And no, I'm not counting "one wears a bow and has eyelashes in an illustration of a book within a comic" as "proof."
 
Hm?

What I mean is that there would be no subconscious assigning of gender identity for a species without any biological impetus for that (ie separate sexes), meaning Yoshis wouldn't have separate genders because they wouldn't have....I don't wanna say the "capacity" for it, but something along those lines. Although it could very well be that Birdo only "thinks he's a girl" because Birdo's species also lacks separate sexes, meaning that they can't really be "boys" or "girls" by simple definition of that they are a single-sex species, and as such lack that sort of differentiation on the bottom line. Which would be less "transgender" and more "conscious adoption of a biologically-inapplicable gender."
 
I feel a species that has lived alongside humans surely have established similar constructs like gender so I find it that explanation just a long route for a short path.
 
Mario Kart is so plotless and casual fun with friends/rivals that in-universe the characters take turns on who gets to win the trophy (while the other racers act as challenges to overcome). They're all expert difficulty by nature but play easy for newby cup challengers.

Also in the manual for SMK it pretty much implies Lakitu is the creator of the event, so I also headcanon that that remains true for the rest of the MK series, he bands the kingdoms together while they're at a truce, with the help of citizens of every kingdom to make the courses and rules.

These 2 things also apply to every plotless Mario Sports game since Lakitu is always the coach/referee sometimes partnered with a Toad.

*edit*
Also I forget if I said this on an earlier page but canon Mario games are essentially Mario & often-Bowser playing the literal game of Capture the Flag, to decide a war. That's why enemies only need to tap Mario to defeat him, and vice versa, (t)he(y) got tagged.
This could explain why enemies are harder for M&L to defeat in M&L series (if we were to assume they're the same bros despite being a spin-off series), in M&L they're having truer fights instead of tagging.

The marioverse is very magic, and so the in-universe games effect the physics of the worlds.
 
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Personally, I find "transgender" to be "subconscious gender assignment gone awry," ie a boy whose mind thinks he's a girl or vice-versa in the case of humans. Adopting gender would be caused by non-biological stimuli, ie non-binary and gender fluidity are not part of the ways human brains can subconsciously develop, but they still exist in nature and as such one can identify with them if they feel more similar to them. Basically, a vaguely similar thing, but not the same thing.

Cats do have separate "genders" by default, as there are different behaviors associated with males and females. For an asexual species to be a "he" or "she," they would need to adopt concepts from a species that does have actual males and females. As for the use of "he," it's a way to be generic for animals since "it" is considered rude in the case of sapient creatures (in fact it's the only pronoun I'd be annoyed if you called me by). Which might explain Birdo's description a bit more (even though that was clearly not the intent at the time).
 
headcanon: the fabrics of the marioverse are infused with happiness chemicals (serotonin, dopamine, etc), creating the zany personalities, rubbery bounciness, and vibrances everybody loves
 
Robo-Mario was a semi-successful to recreate human behaviors. 14 years later, Gooigi is the first truly successful attempt to recreate the behaviors of a human.